Abstract To evaluate the feasibility of using a milled polyetheretherketone (PEEK) post and core in endodontically treated teeth with or without a ferrule. Sixty bovine tooth roots were endodontically treated followed by cementation of intraradicular retainers (IR), according to each experimental group: a) non-ferrule glass fiber post (f0FP); b) 2-mm-ferrule glass fiber post (f2FP); c) non-ferrule resized glass fiber post (f0PR); d) 2-mm-ferrule resized glass fiber post (f2PR); e) non-ferrule PEEK post and core (f0PPC); and f) 2-mm-ferrule PEEK post and core (f2PPC). Metal crowns were made and cemented. A periodontal ligament was simulated using polyether. A force was applied to the palatine portion of each sample at 45°, until fracture. Fracture resistance data were submitted to two-way ANOVA and Tukey’s test (α = 0.05). Three-dimensional digital models were developed to calculate the tensions formed in the root using finite element analysis. Models of glass fiber posts and PEEK posts and cores were evaluated with or without a ferrule. The results were analyzed by the Mohr-Coulomb criterion. The type of IR was not influenced by fracture strength (p = 0.243). There were significant statistical differences among the remaining factors. Ferrule groups had greater fracture resistance, and the failure mode of teeth with a ferrule was more catastrophic than the non-ferrule group. A ferrule increases fracture resistance and influences failure mode; the PEEK post and core did not modify the biomechanics of endodontically treated teeth, and resembled the glass fiber post results. The crack initiation point differed between the ferrule and non-ferrule groups.

Aim: The purpose of this in vitro study was to analyze the influence of nicotine on the extracellular polysaccharides in Fusobacterium nucleatum biofilm. Methods: F. nucleatum (ATCC 10953) biofilms supplemented with different concentrations of nicotine (0, 0.5, 1, 2, 4, and 8 mg/mL) were grown in two different BHI broth conditions [no sucrose and 1% sucrose]. Extracellular polysaccharides assay, pH measurements, and a spectrophotometric assay were performed. Data were submitted for ANOVA and Tukey honestly significant difference analyses (HSD) tests (α =.05). Results: Extracellular polysaccharides synthesis was influenced by an interaction between nicotine concentrations and growth medium solution containing sucrose (P<.05). The pH values declined in the sucrose-exposed biofilm were greater than in the group exposed only to nicotine (P<.05). The biofilm exposed to sucrose and nicotine had a higher total biofilm growth (P<.05) than the nicotine-treated biofilm without sucrose. Conclusions: Regardless of sucrose exposure, biofilms exposed to different nicotine concentrations influenced the amount of extracellular polysaccharides.

Abstract Mucopolysaccharidosis (MPS) is a heterogeneous group of rare, chronic, progressive and systemic inherited disorders resulting from deficiency or lack of lysosomal enzymes responsible for the degradation of glycosaminoglycans. Products of nitrosative stress have been previously detected in blood and urine samples of patients with MPS. However, it is unclear whether they are present in the saliva of MPS patients and also if they correlate with salivary parameters such as flow and pH. This study compared the salivary levels of NOX (NO2- + NO3-), nitrite (NO2-), protein (albumin), erythrocyte and leukocyte numbers, as well as the salivary flow rate and pH values of samples obtained from 10 MPS patients and 10 healthy subjects. MPS patients exhibited higher salivary levels of NOX and NO2- when compared to healthy subjects (p < 0.05). Albumin was only detected in six saliva samples of MPS patients and, erythrocytes and leukocytes were detected in 60% and 40% of the MPS patients, respectively. In addition, salivary flow rate and pH averages were statistically lower in this group when compared to healthy samples (p < 0.05). Overall, the data indicates that the salivary levels of NO products can be used in combination with other heath indicators to monitor MPS disorders.

Resumo O objetivo neste estudo foi avaliar o deslocamento dental em próteses totais incluídas em diferentes tipos de muflas e polimerizadas pelo ciclo australiano ou por micro ondas. Pinos metálicos foram colocados nos dentes como pontos de referência para as medidas: a) Borda incisal dos incisivos centrais maxilares e mandibulares, cúspide vestibular dos primeiros pré molares maxilares e mandibulares e cúspides mésiovestibulares dos segundos molares maxilares e mandibulares; b) Distâncias látero-laterais incisivo a incisivo, pré-molar a pré-molar e molar a molar; c) Distâncias horizontais anteroposterior incisivo esquerdo a molar esquerdo e incisivo direito a molar direito, e d) Vertical. As próteses foram separadas de acordo com o tipo de mufla (n=20): G1- Metálica; G2-HH bi-maxilar metálica; G3- Micro-ondas; G4- HH bi-maxilar micro ondas. Ciclo de polimerização para G1 e G2, água a 65ºC por 30 minutos, aquecimento interrompido por 30 minutos, reaquecimento a 65ºC por 30 minutos, seguido de água fervente por 1 hora. Para G3 e G4, micro ondas (20 minutos a 140 W e 5 minutos a 560 W). As medidas foram avaliadas com microscópio comparador linear. A distância final entre os dentes foi obtida subtraindo o valor da distância antes do processamento da prótese do valor da distância resultante do deslocamento dentário após o processamento. A diferença obtida entre essas distâncias foi submetida à ANOVA de 2 fatores, considerando como variáveis tipo de mufla e tipo de prótese. A prótese maxilar apresentou maior deslocamento dental quando comparada à mandibular. Os tipos de muflas e a interação com os tipos de próteses mostraram movimentos dentais similares antes e depois da polimerização. Em conclusão, o deslocamento dental promovido por diferentes tipos de frascos em próteses curadas por diferentes ciclos de polimerização promoveu efeito semelhante na alteração da distância entre os dentes. Maior valor para a mudança de distância entre os dentes ocorreu na prótese maxilar.

Abstract This study evaluated the dental displacement in dentures included in different flasks and processed by the Australian cycle modified or by microwave. Metal pins were placed on the teeth as reference for measurements: a) Incisal edge of maxillary and mandibular central incisors, buccal cusp of maxillary and mandibular vestibular first premolars, and mesiobuccal maxillary and mandibular cusps of second molars; b) Incisor to incisor, premolar to premolar and molar to molar; c) Left incisor to left molar and right incisor to right molar, and d) Vertical. Dentures were divided according to flasks (n=10): G1- Metal; G2- HH bimaxillary metal; G3-Microwaves; G4- HH bimaxillary microwaves. For G1 and G2, polymerization at 65ºC/30 min, flame quenched for 30 min, heating at 65ºC/30 min, boiling water for 1 h. For G3 and G4, microwave (20 min/140 W and 5 min/560 W). Comparator microscope with digital camera and analytical unit assessed the measurements before and after denture polymerization. The final distance was made subtracting the distance before the denture processing from the distance of the dental displacement after processing. The value of the difference was submitted to 2-way ANOVA, considering the flask type and denture type. Maxillary denture showed greater displacement when compared to mandibular denture. Flask types and interaction with denture types showed similar difference between before and after polymerization. In conclusion, displacement promoted by flask types in dentures cured by polymerization cycles promoted similar effect on the distance between teeth. Greater value for distance between teeth occurred for maxillary denture.

RESUMO O objetivo deste artigo é relatar um caso clínico de reabilitação de incisivos superiores fraturados sem exposição pulpar, com considerações na área de endodontia, periodontia e dentística restauradora. O caso relata um trauma que resultou em perda substancial de tecido dentário em ângulo mesial de ambos os incisivos centrais superiores. Na parede palatina, a fratura se estendeu além do cíngulo até a região subgengival. A cirurgia periodontal foi realizada para restabelecer o espaço biológico. As avaliações clínicas e radiográficas não demonstraram necessidade de tratamento endodôntico, pois a polpa se apresentava vital e sem alterações. Os procedimentos restauradores foram minimamente invasivos através da realização de restauração direta com resina composta. Foi realizado acompanhamento de três anos, consistindo em reavaliação dos aspectos clínicos e radiográficos, polimento das restaurações e documentação fotográfica. Nenhuma alteração do tecido pulpar foi observada após o período de acompanhamento. Os procedimentos restauradores, tecidos adjacentes e vitalidade pulpar foram considerados adequados e a paciente demonstrou satisfação com o tratamento.

ABSTRACT Multiple clinical specialties are usually needed for a successful long-term treatment in buccal cavity. The aim of this article is to report a clinical case of multi-disciplinary rehabilitation of fracture upper incisors without pulp exposure, concerning about endodontics, periodontics and restorative dentistry comments or procedures. A case of a patient reporting trauma that resulted in fracture and substantial loss of hard tissue, in mesial angle of both upper central incisors (11 and 21). In palatal side, fracture extended beyond cingulum up to subgingival region. Periodontal surgery was performed in order to reestablish biological space. Clinical and radiographic assessments demonstrated no need for endodontic treatment, since pulp was vital and non-altered. Preparations for restorative procedures were minimally invasive, followed by composite direct. A three-year follow-up was performed, consisting in re-assessment of clinical and radiographic aspects, re-polishing of the restorations and photographic documentation. No pulp tissue alteration was observed after the follow-up period. Restorative procedures, adjacent tissues and pulp vitality were considered adequate, and the patient was satisfied with the treatment.

Resumo O objetivo neste estudo foi verificar o efeito da diminuição de volume do implante, desajuste vertical entre o pilar e plataforma protética, torque de afrouxamento do parafuso protético e distribuição da tensão no parafuso em pilares de titânio e zircônia. Dez pilares personalizados de cada material foram fresados e conectados aos implantes de titânio. A diminuição de volume do implante foi avaliada com microtomografia, o desajuste vertical com microscopia óptica e o torque de afrouxamento do parafuso protético com chave de torque digital. Todas as análises experimentais foram realizadas antes e após aplicação do ciclo mecânico (1.000.000 ciclos, 100 N/2 Hz). Modelos virtuais das estruturas foram criados para análise por elementos finitos e a tensão no parafuso obtida com valores de von Mises. Os dados foram analisados usando teste t independente, análise de varância dois fatores para medidas repetidas e teste de Tukey HSD (a=0,05). Não houve diferença significativa na diminuição de volume do implante para os dois materiais do pilar (p= 0,662). Os pilares de titânio proporcionaram maiores valores de torque de afrouxamento após o ciclo mecânico (p<0,001). O menor desajuste marginal foi obtido com os pilares de titânio antes e após o ciclo mecânico (p<0,001). A distribuição da tensão no parafuso foi similar entre os materiais. Em conclusão, os pilares personalizados de titânio reduziram o desajuste marginal e aumentaram a manutenção do torque dos parafusos protéticos quando comparados aos pilares de zircônia.

Abstract The aim of this study was to verify the effect of the implant volume loss, vertical misfit between abutment and prosthetic platform, prosthetic screw loosening torque, and screw stress distribution in titanium and zirconia abutments. Ten CAD/CAM system custom abutments of each material were milled and attached to the titanium implants. The implant volume loss was evaluated by microtomography, the vertical misfit with optical microscopy, and digital torque wrench measured the prosthetic screw loosening. All experimental analyses were performed before and after mechanical cycle (1,000,000 cycles, 100 N/2 Hz). Virtual models of the structures were created for finite element analysis, and the stress on the screw obtained with von Mises procedure. Data were analyzed using an independent t-test, two-way ANOVA for repeated measures, and Tukey’s HSD test (a=0.05). There was no significant difference in the implant volume loss for the two abutment materials (p=0.662). Titanium abutments provided higher loosening torque values after mechanical cycling (p<0.001). Lesser marginal misfit was obtained with titanium abutments before and after mechanical cycling (p<0.001). The stress distribution on the screw was similar between abutment materials. In conclusion, CAD/CAM custom titanium abutment reduced the marginal misfit and increased the torque maintenance of prosthetic screws when compared to CAD/CAM custom zirconia abutment.